< COLOR=#FF0000>1996 Science Teachers Association of Texas (STAT) Convention
For an excellent science experiment, I would like to suggest that you use Schramm and Hestrin Medium. The formula is as follows:
2% (w/v) glucose; 0.5% (w/v) peptone (Difco Bactopeptone); 0.5% (w/v) yeast extract (Difco); 0.27% anhydrous disodium phosphate; and 0.15% (w/v) citric acid monohydrate. Adjust the pH to around 5.0 using 1 N acetic acid. Solidify using Difco Bacto Agar (1.5%) and autoclave for 15 min at 15 lb/square inch pressure. Let the agar cool but not too much and pour into sterile disposable plastic Petri dishes (100 mm) while still warm and molten. After solidifaction, seal the dishes using Parafilm strips and store upside down to prevent condensation on the agar surface. Store in the refrigerator until ready for use.
After streaking with the field sample, incubate at 25-30 degrees C for about 3-4 days until colonies appear. Then pick individual colonies and transfer into the liquid Schramm and Hestrin medium in tubes. Dispose of the used plates by autoclaving.
DISCLAIMER- This recipe is given and disclosed as advice only, and I am not responsible for any mis-use or abuse of the procedures and/or organisms which may grow in the medium and cause harm or damage to humans or life. These experiments should be performed under the guidance of a science teacher who has knowledge of laboratory methods in microbiology.
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