147. Abstract

An overview of the synthesis and uses of the photoaffinity analog 5-azido-UDP-glucose in the study of -glucan synthases from cotton and other systems is presented. When combined with protein purification techniques and enzymatic activity assays, photoaffinity labeling with [-³²P]5-azido-UDP-glucose has allowed the identification of the UDP-glucose catalytic subunits of cellulose synthase from Acetobacter xylinum and Gossypium hirsutum. An 83 kDa protein was identified as the cellulose synthase from Acetobacter xylinum preparations. A 37 kDa protein has been identified as a component of cellulose synthase from cotton, as well as a 52 kDa protein identified as being involved in callose biosynthesis. The criteria by which these proteins were identified and how this type of photoaffinity approach can be utilized in the study of cellulose biosynthesis are discussed.

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Last modified 21 March 2008.
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