| | | The movie above shows the tilt of a freeze-fractured yeast cell. Before the movie begins, you have
no idea of depth perception; however, when the movie starts, you will immediately use your depth perception cues and be able
to easily discern convex structures from concave structure. Notice the protruding portion of a membrane in the lower left corner.
The large, convex structure in the is the nucleus, with its prominent nuclear pores. There should be several
concave depressions on the nucleus. Note also the prominent tear of the freeze fracture replica protruding 90 degrees
above the replica surface. This series of speicmen tilts was made on our Philips 420 TEM which has a eucentric goniometer
stage which can tilt +- 60 degrees.
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